General Information


DRAVP ID  DRAVPe01393

Peptide Name   UL54(1221-1242)

Sequence  LPRRLHLEPAFLPYSVKAHECC

Sequence Length  22

UniProt ID  P08546 

Source  Synthetic construct(derived from human cytomegalovirus(HCMV) UL54 protein)



Activity Information


Target Organism  HCMV

Assay  ELISA

Activity 

  • [Ref.12857903]human cytomegalovirus(HCMV):inhibition of phyical interaction between UL54 and UL44(IC50=11 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  DNA polymerase

Mechanism  Peptide derived from C-terminus of UL54 can block the physical interaction between UL54 and UL44,thus inhibits the activity of DNA polymerase.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01393

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C117H183N33O29S2

Absent amino acids  DGIMNQTW

Common amino acids  L

Mass  2580.06

Pl  8.06

Basic residues  5

Acidic residues  2

Hydrophobic residues  8

Net charge  3

Boman Index  -2899

Hydrophobicity  -10.91

Aliphatic Index  93.18

Half Life 

  •     Mammalian:5.5 hour
  •     Yeast:3 min
  •     E.coli:2 min

Extinction Coefficient cystines  1615

Absorbance 280nm  76.9

Polar residues  4



Literature Information


Literature 1

Title   Inhibition of human cytomegalovirus DNA polymerase by C-terminal peptides from the UL54 subunit.

Pubmed ID   12857903

Reference   J Virol. 2003 Aug;77(15):8336-44.

Author   Loregian A, Rigatti R, Murphy M, Schievano E, Palu G, Marsden HS.

DOI   10.1128/jvi.77.15.8336-8344.2003