General Information


DRAVP ID  DRAVPe01425

Peptide Name   Pcr-400

Sequence  CCFLNITNSHVSILQERPPLENRVLTGWGL

Sequence Length  30

UniProt ID  P14075 

Source  Synthetic construct(derived from HTLV-1 envelope glycoprotein (gp21))



Activity Information


Target Organism  HTLV

Assay  Luciferase assay

Activity 

  • [Ref.19114713]:Human T cell leukemia virus type 1(HTLV-1):inhibition of mambrane fusion in 293T cells(IC50=0.18 ± 0.01 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  mambrane

Mechanism  The peptide is an effective inhibitor of HTLV-1 envelope-catalyzed membrane fusion and thus inhibits virus entry.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01425

Linear/Cyclic  Linear

N-terminal Modification  Acetylation

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C151H241N43O43S2

Absent amino acids  ADKMY

Common amino acids  L

Mass  3410.96

Pl  6.74

Basic residues  3

Acidic residues  2

Hydrophobic residues  11

Net charge  1

Boman Index  -3325

Hydrophobicity  10.33

Aliphatic Index  110.33

Half Life 

  •     Mammalian:1.2 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  5625

Absorbance 280nm  193.97

Polar residues  11



Literature Information


Literature 1

Title   Basic residues are critical to the activity of peptide inhibitors of human T cell leukemia virus type 1 entry.

Pubmed ID   19114713

Reference   J Biol Chem. 2009 Mar 6;284(10):6575-84. 

Author   Lamb D, Mirsaliotis A, Kelly SM, Brighty DW.

DOI   10.1074/jbc.M806725200