General Information

General Information

DRAVP ID DRAVPe01507

Peptide Name GBVA10-9(P16L)

Sequence CWVRLGRYLLRRLKTLFT

Sequence Length 18

UniProt ID No entry found

Source Synthetic construct(derived from GBVA non-structutal protein 5A)

Activity Information

Target Organism HCV

Assay qRT-PCR assay

Activity

[Ref.23175359]hepatitis C virus(Jc1-Luc HCVcc):inhibition of virus entry in Huh7.5.1 cells(IC50=0.16 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.23175359]HepG2 cell:LC50=90 μM(4h of treatment);
HeLa cell:LC50=40 μM(4h of treatment);
Huh7.5.1 cell:LC50=90 μM(4h of treatment);LC50=50 μM(24h of treatment).

Binding Target Not found

Mechanism GBVA10-9 can inhibit virus entry at a postattachment step.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01507

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C107H176N32O22S

Absent amino acids ADEHIMNPQS

Common amino acids L

Mass 2294.84

Pl 11.56

Basic residues 5

Acidic residues 0

Hydrophobic residues 8

Net charge 5

Boman Index -3434

Hydrophobicity 14.44

Aliphatic Index 124.44

Half Life

Mammalian:1.2 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 6990

Absorbance 280nm 411.18

Polar residues 5

Literature Information

Literature 1

Title Screening and rational design of hepatitis C virus entry inhibitory peptides derived from GB virus A NS5A.

Pubmed ID 23175359

Reference J Virol. 2013 Feb;87(3):1649-57.

Author Liu X, Huang Y, Cheng M, Pan L, Si Y, Li G, Niu Y, Zhao L, Zhao J, Li X, Chen Y, Yang W.

DOI 10.1128/JVI.02201-12