General Information

General Information

DRAVP ID DRAVPe01525

Peptide Name Temporin B

Sequence LLPIVGNLLKSLL

Sequence Length 13

UniProt ID P79874

Source Rana temporaria(European common frog)

Activity Information

Target Organism HSV

Assay

Activity

[Ref.29483113]Herpes simplex virus 1 (HSV-1):viral titer inhibition on Vero cells(IC50=2.507 μg/ml);inhibition of HSV-1 replication in HeLa S3 cells(IC50=93 μg/ml);inhibition of HSV-1 replication in HEK-293 cells(IC50=84 μg/ml).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.29483113]Vero cells:CC50=90.4μg/ml;
no toxicity was observed at concentrations as high as 50 μg/ml against HeLa S3 and HEK-293 cells.

Binding Target envelope

Mechanism Temporin B could disrupt the envelope of virus,and partially affected different stages of the HSV-1 life cycle, including the attachment and the entry of the virus into the host cell, as well as the subsequent postinfection phase.

Structure Information

PDB ID 6GIL

Predicted Structure Download DRAVPe01525

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C67H121N15O16

Absent amino acids ACDEFHMQRTWY

Common amino acids L

Mass 1392.79

Pl 8.75

Basic residues 1

Acidic residues 0

Hydrophobic residues 8

Net charge 1

Boman Index 2383

Hydrophobicity 163.85

Aliphatic Index 232.31

Half Life

Mammalian:5.5 hour
Yeast:3 min
E.coli:2 min

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 3

Literature Information

Literature 1

Title The Amphibian Antimicrobial Peptide Temporin B Inhibits In Vitro Herpes Simplex Virus 1 Infection.

Pubmed ID 29483113

Reference Antimicrob Agents Chemother. 2018 Apr 26;62(5):e02367-17.

Author Marcocci ME, Amatore D, Villa S, Casciaro B, Aimola P, Franci G, Grieco P, Galdiero M, Palamara AT, Mangoni ML, Nencioni L.

DOI 10.1128/AAC.02367-17