General Information


DRAVP ID  DRAVPe01527

Peptide Name   Temporin-SHa (temporin SHa; Temporin-1Sa, Temp-1Sa; XXA; UCLL1c; natural AMPs; frog, amphibians, animals)

Sequence  FLSGIVGMLGKLF

Sequence Length  13

UniProt ID  B3KYH4 

Source  Pelophylax saharica, North Africa



Activity Information


Target Organism  HSV

Assay 

Activity 

  • [Ref.30669255]HSV-1:inhibition of HSV-1 replication in human keratinocytes(52% inhibition at 10 µM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.30669255]Significant cytotoxicity was observed on Keratinocyte at the concentration of 20 μM with a cell viability of 77%

Binding Target  Not found

Mechanism  No machanism information found in the reference(s) presented in this entry



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01527

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C67H108N14O15S

Absent amino acids  ACDEHNPQRTWY

Common amino acids  GL

Mass  1381.74

Pl  8.75

Basic residues  1

Acidic residues  0

Hydrophobic residues  7

Net charge  1

Boman Index  2590

Hydrophobicity  166.92

Aliphatic Index  142.31

Half Life 

  •     Mammalian:1.1 hour
  •     Yeast:3 min
  •     E.coli:2 min

Extinction Coefficient cystines  0

Absorbance 280nm  0

Polar residues  4



Literature Information


Literature 1

Title   Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K³]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1.

Pubmed ID   30669255

Reference   Viruses. 2019 Jan 18;11(1):77.

Author   Roy M, Lebeau L, Chessa C, Damour A, Ladram A, Oury B, Boutolleau D, Bodet C, Lévêque N. 

DOI   10.3390/v11010077