General Information


DRAVP ID  DRAVPe01528

Peptide Name   Temporin-Sha[K3]

Sequence  FLKGIVGMLGKLF

Sequence Length  13

UniProt ID  No entry found

Taxon ID  None

Source  Synthetic construct(derived from Temporin-SHa)

Validation   Experimentally Validated



Origin Information


Gene Name/ID  Not Available

GenBank  Not Available

Amino Acid position  Not Available

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not Available



Activity Information


Target Organism  HSV

Assay 

Activity 

  • [Ref.30669255]HSV-1:inhibition of HSV-1 replication in human keratinocytes(48% inhibition at 2.5 µM,57% inhibition at 5 µM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.30669255]Significant cytotoxicity was observed on Keratinocyte at the concentration of 10 μM with a cell viability of 74%.

Binding Target  Not found

Mechanism  No machanism information found in the reference(s) presented in this entry



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01528

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C70H115N15O14S

Absent amino acids  ACDEHNPQRSTWY

Common amino acids  GL

Mass  1422.84

Pl  10

Basic residues  2

Acidic residues  0

Hydrophobic residues  7

Net charge  2

Boman Index  2375

Hydrophobicity  143.08

Aliphatic Index  142.31

Half Life 

  •     Mammalian:1.1 hour
  •     Yeast:3 min
  •     E.coli:2 min

Extinction Coefficient cystines  0

Absorbance 280nm  0

Polar residues  3



Literature Information


Literature 1

Title   Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K³]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1.

Pubmed ID   30669255

Reference   Viruses. 2019 Jan 18;11(1):77.

Author   Roy M, Lebeau L, Chessa C, Damour A, Ladram A, Oury B, Boutolleau D, Bodet C, Lévêque N. 

DOI   10.3390/v11010077