General Information


DRAVP ID  DRAVPe01551

Peptide Name   Temporin G

Sequence  FFPVIGRILNGIL

Sequence Length  13

UniProt ID  P79875 

Source  European common frog, Rana temporaria



Activity Information


Target Organism  influenza virus,Sendai virus

Assay 

Activity 

  • [Ref.33538061]influenza virus:inhibition of replication in A549 cells(IC50=13 μM);
  • Sendai virus:inhibit the replication of Sendai virus in A549 cells.

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.33538061]A549 cells:CC50=73 μM;

Binding Target  Not found

Mechanism  Temporin G block the conformational rearrangements of HA2 subunit, which are essential for the viral envelope fusion with intracellular endocytic vesicles, thereby neutralizing the virus entry into the host cell.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01551

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C72H115N17O15

Absent amino acids  ACDEHKMQSTWY

Common amino acids  I

Mass  1458.81

Pl  9.75

Basic residues  1

Acidic residues  0

Hydrophobic residues  8

Net charge  1

Boman Index  1492

Hydrophobicity  157.69

Aliphatic Index  172.31

Half Life 

  •     Mammalian:1.1 hour
  •     Yeast:3 min
  •     E.coli:2 min

Extinction Coefficient cystines  0

Absorbance 280nm  0

Polar residues  3



Literature Information


Literature 1

Title   Temporin G, an amphibian antimicrobial peptide against influenza and parainfluenza respiratory viruses: Insights into biological activity and mechanism of action. 

Pubmed ID   33538061

Reference   FASEB J. 2021 Feb;35(2):e21358. 

Author   De Angelis M, Casciaro B, Genovese A, Brancaccio D, Marcocci ME, Novellino E, Carotenuto A, Palamara AT, Mangoni ML, Nencioni L. 

DOI   10.1096/fj.202001885RR