General Information


DRAVP ID  DRAVPe01555

Peptide Name   Dermaseptin-4 (DS IV; Dermaseptin-S4, DS4; Frogs, amphibians, animals)

Sequence  ALWMTLLKKVLKAAAKAALNAVLVGANA

Sequence Length  28

UniProt ID  P80280 

Source  Phyllomedusa sauvagei (Sauvage's leaf frog)



Activity Information


Target Organism  HSV,HIV

Assay  Chemiluminescence Reporter Gene Assay

Activity 

  • [Ref.23161023]HSV-2 acyclovir-sensitive strain(911411):inhibition the cytopathic effect(CPE) of HSV-2 in Vero cells(EC50=2.10 μM);
  • HSV-2 acyclovir-resistant strain(ROI2):inhibition of cytopathic effet(CPE) of HSV-2 in Vero cells(EC50=6 μM).
  • [Ref.15780876]human immunodeficiency virus type 1 (HIV-1):Inhibition of viral infection in Vero P4-CCR5 cells(IC50=2 µM).

Hemolytic Activity 

  • [Ref:11850249]IC50=20 μM against human erythrocytes

Cytotoxicity 

  • [Ref.23161023]Vero cells:CC50=7.50 μM.
  • [Ref.15780876]P4-CCR5 cells:CC50=4.5 μM.

Binding Target  viral membrane

Mechanism  exerts a selective activity on viral particles and disturbs their organization by breaking the viral membrane, leading to the exposure of HIV-1 core and its dissociation. 



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01555

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C132H229N35O32S

Absent amino acids  CDEFHIPQRSY

Common amino acids  A

Mass  2850.55

Pl  10.48

Basic residues  4

Acidic residues  0

Hydrophobic residues  19

Net charge  4

Boman Index  2550

Hydrophobicity  103.21

Aliphatic Index  146.79

Half Life 

  •     Mammalian:4.4 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  5500

Absorbance 280nm  203.7

Polar residues  4



Literature Information


Literature 1

Title   Antibacterial properties of dermaseptin S4 derivatives with in vivo activity.

Pubmed ID   11850249

Reference   Antimicrob Agents Chemother. 2002 Mar;46(3):689-694.

Author   Navon-Venezia S, Feder R, Gaidukov L, Carmeli Y, Mor A.

DOI   10.1128/AAC.46.3.689-694.2002

Literature 2

Title   In vitro antiviral activity of dermaseptin S(4) and derivatives from amphibian skin against herpes simplex virus type 2.

Pubmed ID   23161023

Reference   J Med Virol. 2013 Feb;85(2):272-81.

Author   Bergaoui I, Zairi A, Tangy F, Aouni M, Selmi B, Hani K.

DOI   10.1002/jmv.23450

Literature 3

Title   The antimicrobial peptide dermaseptin S4 inhibits HIV-1 infectivity in vitro.

Pubmed ID   15780876

Reference   Virology. 2005 Apr 10;334(2):264-75.

Author   Lorin C, Saidi H, Belaid A, Zairi A, Baleux F, Hocini H, Bélec L, Hani K, Tangy F.

DOI   10.1016/j.virol.2005.02.002