General Information

General Information

DRAVP ID DRAVPe01555

Peptide Name Dermaseptin-4 (DS IV; Dermaseptin-S4, DS4; Frogs, amphibians, animals)

Sequence ALWMTLLKKVLKAAAKAALNAVLVGANA

Sequence Length 28

UniProt ID P80280

Taxon ID None

Source Phyllomedusa sauvagei (Sauvage's leaf frog)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism HSV,HIV

Assay Chemiluminescence Reporter Gene Assay

Activity

[Ref.23161023]HSV-2 acyclovir-sensitive strain(911411):inhibition the cytopathic effect(CPE) of HSV-2 in Vero cells(EC50=2.10 μM);
HSV-2 acyclovir-resistant strain(ROI2):inhibition of cytopathic effet(CPE) of HSV-2 in Vero cells(EC50=6 μM).
[Ref.15780876]human immunodeficiency virus type 1 (HIV-1):Inhibition of viral infection in Vero P4-CCR5 cells(IC50=2 µM).

Hemolytic Activity

[Ref:11850249]IC50=20 μM against human erythrocytes

Cytotoxicity

[Ref.23161023]Vero cells:CC50=7.50 μM.
[Ref.15780876]P4-CCR5 cells:CC50=4.5 μM.

Binding Target viral membrane

Mechanism exerts a selective activity on viral particles and disturbs their organization by breaking the viral membrane, leading to the exposure of HIV-1 core and its dissociation.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01555

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C132H229N35O32S

Absent amino acids CDEFHIPQRSY

Common amino acids A

Mass 2850.55

Pl 10.48

Basic residues 4

Acidic residues 0

Hydrophobic residues 19

Net charge 4

Boman Index 2550

Hydrophobicity 103.21

Aliphatic Index 146.79

Half Life

Mammalian:4.4 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5500

Absorbance 280nm 203.7

Polar residues 4

Literature Information

Literature 1

Title Antibacterial properties of dermaseptin S4 derivatives with in vivo activity.

Pubmed ID 11850249

Reference Antimicrob Agents Chemother. 2002 Mar;46(3):689-694.

Author Navon-Venezia S, Feder R, Gaidukov L, Carmeli Y, Mor A.

DOI 10.1128/AAC.46.3.689-694.2002

Literature 2

Title In vitro antiviral activity of dermaseptin S(4) and derivatives from amphibian skin against herpes simplex virus type 2.

Pubmed ID 23161023

Reference J Med Virol. 2013 Feb;85(2):272-81.

Author Bergaoui I, Zairi A, Tangy F, Aouni M, Selmi B, Hani K.

DOI 10.1002/jmv.23450

Literature 3

Title The antimicrobial peptide dermaseptin S4 inhibits HIV-1 infectivity in vitro.

Pubmed ID 15780876

Reference Virology. 2005 Apr 10;334(2):264-75.

Author Lorin C, Saidi H, Belaid A, Zairi A, Baleux F, Hocini H, Bélec L, Hani K, Tangy F.

DOI 10.1016/j.virol.2005.02.002