General Information

General Information

DRAVP ID DRAVPe01555

Peptide Name Dermaseptin-4 (DS IV; Dermaseptin-S4, DS4; Frogs, amphibians, animals)

Sequence ALWMTLLKKVLKAAAKAALNAVLVGANA

Sequence Length 28

UniProt ID P80280

Source Phyllomedusa sauvagei (Sauvage's leaf frog)

Activity Information

Target Organism HSV,HIV

Assay Chemiluminescence Reporter Gene Assay

Activity

[Ref.23161023]HSV-2 acyclovir-sensitive strain(911411):inhibition the cytopathic effect(CPE) of HSV-2 in Vero cells(EC50=2.10 μM);
HSV-2 acyclovir-resistant strain(ROI2):inhibition of cytopathic effet(CPE) of HSV-2 in Vero cells(EC50=6 μM).
[Ref.15780876]human immunodeficiency virus type 1 (HIV-1):Inhibition of viral infection in Vero P4-CCR5 cells(IC50=2 µM).

Hemolytic Activity

[Ref:11850249]IC50=20 μM against human erythrocytes

Cytotoxicity

[Ref.23161023]Vero cells:CC50=7.50 μM.
[Ref.15780876]P4-CCR5 cells:CC50=4.5 μM.

Binding Target viral membrane

Mechanism exerts a selective activity on viral particles and disturbs their organization by breaking the viral membrane, leading to the exposure of HIV-1 core and its dissociation.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01555

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C132H229N35O32S

Absent amino acids CDEFHIPQRSY

Common amino acids A

Mass 2850.55

Pl 10.48

Basic residues 4

Acidic residues 0

Hydrophobic residues 19

Net charge 4

Boman Index 2550

Hydrophobicity 103.21

Aliphatic Index 146.79

Half Life

Mammalian:4.4 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5500

Absorbance 280nm 203.7

Polar residues 4

Literature Information

Literature 1

Title Antibacterial properties of dermaseptin S4 derivatives with in vivo activity.

Pubmed ID 11850249

Reference Antimicrob Agents Chemother. 2002 Mar;46(3):689-694.

Author Navon-Venezia S, Feder R, Gaidukov L, Carmeli Y, Mor A.

DOI 10.1128/AAC.46.3.689-694.2002

Literature 2

Title In vitro antiviral activity of dermaseptin S(4) and derivatives from amphibian skin against herpes simplex virus type 2.

Pubmed ID 23161023

Reference J Med Virol. 2013 Feb;85(2):272-81.

Author Bergaoui I, Zairi A, Tangy F, Aouni M, Selmi B, Hani K.

DOI 10.1002/jmv.23450

Literature 3

Title The antimicrobial peptide dermaseptin S4 inhibits HIV-1 infectivity in vitro.

Pubmed ID 15780876

Reference Virology. 2005 Apr 10;334(2):264-75.

Author Lorin C, Saidi H, Belaid A, Zairi A, Baleux F, Hocini H, Bélec L, Hani K, Tangy F.

DOI 10.1016/j.virol.2005.02.002