General Information

General Information

DRAVP ID DRAVPe01559

Peptide Name Retrocyclin-1 (RC1)

Sequence GICRCICGRGICRCICGR

Sequence Length 18

UniProt ID No entry found

Source Synthetic construct

Other Link DRAVPa0039

Activity Information

Target Organism HIV

Assay

Activity

[Ref.15210812]HIV-1 A:inhibition of infection in JC53-BL cells(IC50=5.51 ± 1.13 µg/ml);
HIV-1 B:inhibition of infection in JC53-BL cells(IC50=4.15± 0.99 µg/ml);
HIV-1 CRF01_AE:inhibition of infection in JC53-BL cells(IC50=5.33± 0.80 µg/ml);
HIV-1 D:inhibition of infection in JC53-BL cells(IC50=4.57 ± 0.53 µg/ml);
HIV-1 G:inhibition of infection in JC53-BL cells(IC50=5.86 ± 0.95 µg/ml);
HIV-1 BF:inhibition of infection in JC53-BL cells(IC50=7.24 µg/ml).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity No cytotoxicity information found in the reference(s) presented

Binding Target gp120,CD4

Mechanism The peptide binds to gp120 and CD4, thereby inhibiting HIV entry.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01559

Linear/Cyclic Cyclic

N-terminal Modification Free

C-terminal Modification Free

Other Modification Disulfide bonds between Cys3 and Cys16,Cys5 and Cys14,Cys7 and Cys12.

Stereochemistry L

Physicochemical Information

Formula C74H136N30O19S6

Absent amino acids ADEFHKLMNPQSTVWY

Common amino acids C

Mass 1942.44

Pl 9.01

Basic residues 4

Acidic residues 0

Hydrophobic residues 4

Net charge 4

Boman Index -2856

Hydrophobicity 74.44

Aliphatic Index 86.67

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 375

Absorbance 280nm 22.06

Polar residues 10

Literature Information

Literature 1

Title Retrocyclin: a primate peptide that protects cells from infection by T- and M-tropic strains of HIV-1

Pubmed ID 15210812

Reference J Immunol. 2004 Jul 1;173(1):515-20.

Author Wang W, Owen SM, Rudolph DL, Cole AM, Hong T, Waring AJ, Lal RB, Lehrer RI.

DOI 10.4049/jimmunol.173.1.515