General Information

General Information

DRAVP ID DRAVPe01598

Peptide Name Rev (34-50)

Sequence TRQARRNRRRRWRERQR

Sequence Length 17

UniProt ID P05866

Source Synthetic construct

Activity Information

Target Organism HIV

Assay MAGI assay

Activity

[Ref.20580677]HIV-1 IIIB:inhibition of virus replication in PM1-CCR5 cells(EC50=1.7±0.25 μM);
HIV-1 Bal:inhibition of virus replication in PM1-CCR5 cells(EC50>10 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target Not found

Mechanism Peptide derived from the RNA-binding domain of HIV-1 Rev can act as dual-target inhibitors that inhibit HIV-1 entry and viral production in the early and late phases of replication.

Structure Information

PDB ID 1ULL

Predicted Structure Download DRAVPe01598

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C97H173N51O24

Absent amino acids CDFGHIKLMPSVY

Common amino acids R

Mass 2437.77

Pl 12.6

Basic residues 10

Acidic residues 1

Hydrophobic residues 2

Net charge 9

Boman Index -17216

Hydrophobicity -345.88

Aliphatic Index 5.88

Half Life

Mammalian:7.2 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5500

Absorbance 280nm 343.75

Polar residues 2

Literature Information

Literature 1

Title Rev-derived peptides inhibit HIV-1 replication by antagonism of Rev and a co-receptor, CXCR4.

Pubmed ID 20580677

Reference Int J Biochem Cell Biol. 2010 Sep;42(9):1482-8.

Author Shimane K, Kodama EN, Nakase I, Futaki S, Sakurai Y, Sakagami Y, Li X, Hattori T, Sarafianos SG, Matsuoka M.

DOI 10.1016/j.biocel.2010.05.005