General Information

General Information

DRAVP ID DRAVPe01600

Peptide Name Rev (34-50) -A4C-RTD

Sequence TRQARRNRRRRWRERQRAAAACYGRKKRRQRRR

Sequence Length 33

UniProt ID P05866 P04612

Source Synthetic construct

Activity Information

Target Organism HIV

Assay MAGI assay

Activity

[Ref.20580677]HIV-1 IIIB:inhibition of virus replication in PM1-CCR5 cells(EC50=0.37±0.09 μM);
HIV-1 Bal:inhibition of virus replication in PM1-CCR5 cells(EC50>10 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target Not found

Mechanism Peptide derived from the RNA-binding domain of HIV-1 Rev can act as dual-target inhibitors that inhibit HIV-1 entry and viral production in the early and late phases of replication.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01600

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C176H314N88O42S

Absent amino acids DFHILMPSV

Common amino acids R

Mass 4367.05

Pl 12.48

Basic residues 18

Acidic residues 1

Hydrophobic residues 6

Net charge 17

Boman Index -26900

Hydrophobicity -270

Aliphatic Index 15.15

Half Life

Mammalian:7.2 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 6990

Absorbance 280nm 218.44

Polar residues 5

Literature Information

Literature 1

Title Rev-derived peptides inhibit HIV-1 replication by antagonism of Rev and a co-receptor, CXCR4.

Pubmed ID 20580677

Reference Int J Biochem Cell Biol. 2010 Sep;42(9):1482-8.

Author Shimane K, Kodama EN, Nakase I, Futaki S, Sakurai Y, Sakagami Y, Li X, Hattori T, Sarafianos SG, Matsuoka M.

DOI 10.1016/j.biocel.2010.05.005