General Information

General Information

DRAVP ID DRAVPe01604

Peptide Name CCR5 ECL2 (168-191)

Sequence RSQKEGLHYTCSSHFPYSQYQFWK

Sequence Length 24

UniProt ID P51681

Source Synthetic construct

Activity Information

Target Organism HIV

Assay HIV neutralization assay

Activity

[Ref.22403408]HIV-1 YU2:inhibition of virus infection in TZM-bl cells(IC50=136 ± 49 μM);
HIV-1 BaL26:inhibition of virus infection in TZM-bl cells(IC50=138± 36 μM);
HIV-1 HxB2:inhibition of virus infection in TZM-bl cells(IC50=89±31 μM);
HIV-1 NL4-3:inhibition of virus infection in TZM-bl cells(IC50=103±37 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target gp120

Mechanism The peptide binds gp120 and inhibits virus entry.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01604

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C138H191N37O38S

Absent amino acids ADIMNV

Common amino acids S

Mass 3008.32

Pl 9.05

Basic residues 5

Acidic residues 1

Hydrophobic residues 4

Net charge 4

Boman Index -5993

Hydrophobicity -131.25

Aliphatic Index 16.25

Half Life

Mammalian:1 hour
Yeast:2 min
E.coli:2 min

Extinction Coefficient cystines 9970

Absorbance 280nm 433.48

Polar residues 10

Literature Information

Literature 1

Title Peptides from second extracellular loop of C-C chemokine receptor type 5 (CCR5) inhibit diverse strains of HIV-1.

Pubmed ID 22403408

Reference J Biol Chem. 2012 Apr 27;287(18):15076-86.

Author Dogo-Isonagie C, Lam S, Gustchina E, Acharya P, Yang Y, Shahzad-ul-Hussan S, Clore GM, Kwong PD, Bewley CA.

DOI 10.1074/jbc.M111.332361