General Information

General Information

DRAVP ID DRAVPe01605

Peptide Name CCR5 ECL2 (178-191)

Sequence CSSHFPYSQYQFWK

Sequence Length 14

UniProt ID P51681

Source Synthetic construct

Activity Information

Target Organism HIV

Assay HIV neutralization assay

Activity

[Ref.22403408]HIV-1 YU2:inhibition of virus infection in TZM-bl cells(IC50=28 ±7 μM);
HIV-1 BaL26:inhibition of virus infection in TZM-bl cells(IC50=65± 3 μM);
HIV-1 HxB2:inhibition of virus infection in TZM-bl cells(IC50=54±2 μM);
HIV-1 NL4-3:inhibition of virus infection in TZM-bl cells(IC50=53±2 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target gp120

Mechanism The peptide binds gp120 and inhibits virus entry.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01605

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C86H110N20O22S

Absent amino acids ADEGILMNRTV

Common amino acids S

Mass 1808

Pl 8.18

Basic residues 2

Acidic residues 0

Hydrophobic residues 3

Net charge 2

Boman Index -2220

Hydrophobicity -96.43

Aliphatic Index 0

Half Life

Mammalian:1.2 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 8480

Absorbance 280nm 652.31

Polar residues 6

Literature Information

Literature 1

Title Peptides from second extracellular loop of C-C chemokine receptor type 5 (CCR5) inhibit diverse strains of HIV-1.

Pubmed ID 22403408

Reference J Biol Chem. 2012 Apr 27;287(18):15076-86.

Author Dogo-Isonagie C, Lam S, Gustchina E, Acharya P, Yang Y, Shahzad-ul-Hussan S, Clore GM, Kwong PD, Bewley CA.

DOI 10.1074/jbc.M111.332361