DRAVP ID  DRAVPe01637

Peptide Name   DENV Envelope glycoprotein (25-41)

Sequence  LEHGSCVTTMAKDKPTL

Sequence Length  17

UniProt ID  Q5UB51  P17763 

Source  Synthetic construct(derived from DENV Envelope glycoprotein)

Target Organism  DENV

Assay  Focus-forming assay (FFA)

Activity 

• [Ref.30508603]DENV-1:inhibition of virus infection in Vero cells(58%±2.55% inhibition at 50 μM,IC50=10 μM);
• DENV-2:inhibition of virus infection in Vero cells(IC50=10 μM);
• DENV-3:inhibition of virus infection in Vero cells(60% inhibition at 20μM);
• DENV-4:inhibition of virus infection in Vero cells(55% inhibition at 20μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.30508603]<20% cytotoxicity against Vero and C6/36 cells at 200 μM.

Binding Target  E protein

Mechanism  The peptide acts as inhibitors by binding to the E protein, suppressing its conformational changes, and preventing its binding to the cellular receptor, thereby inhibiting viral entry.

PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L

Literature 1

Title   In vitro analysis of synthetic peptides in blocking the entry of dengue virus.

Pubmed ID   30508603

Reference   Virus Res. 2019 Jan 15;260:142-150.

Author   John AM, Jittmittraphap A, Chattanadee S, Alwin Prem Anand A, Shenbagarathai R, Leaungwutiwong P.

DOI   10.1016/j.virusres.2018.11.016