DRAVP ID  DRAVPe01639

Peptide Name   DENV Envelope glycoprotein (340 – 354)

Sequence  GQGKAHNGRLITANP

Sequence Length  15

UniProt ID  Q5UB51  P17763 

Taxon ID  None

Source  Synthetic construct(derived from DENV Envelope glycoprotein)

Validation   Experimentally Validated

Gene Name/ID  Not Available

GenBank  Not Available

Amino Acid position  Not Available

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not available

Target Organism  DENV

Assay  Focus-forming assay (FFA)

Activity 

• [Ref.30508603]DENV-1:inhibition of virus infection in Vero cells(83%±3.72% inhibition at 50 μM,IC50=33 μM);
• DENV-2:inhibition of virus infection in Vero cells(IC50=10 μM);
• DENV-3:inhibition of virus infection in Vero cells(60% inhibition at 20μM);
• DENV-4:inhibition of virus infection in Vero cells(55% inhibition at 20μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.30508603]<20% cytotoxicity against Vero and C6/36 cells at 200 μM.

Binding Target  E protein

Mechanism  The peptide acts as inhibitors by binding to the E protein, suppressing its conformational changes, and preventing its binding to the cellular receptor, thereby inhibiting viral entry.

PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L

Literature 1

Title   In vitro analysis of synthetic peptides in blocking the entry of dengue virus.

Pubmed ID   30508603

Reference   Virus Res. 2019 Jan 15;260:142-150.

Author   John AM, Jittmittraphap A, Chattanadee S, Alwin Prem Anand A, Shenbagarathai R, Leaungwutiwong P.

DOI   10.1016/j.virusres.2018.11.016