General Information

General Information

DRAVP ID DRAVPe01760

Peptide Name 2019-nCoV-HR2P(SARS-CoV-2-S(1168-1203),SARS-HR2P)

Sequence DISGINASVVNIQKEIDRLNEVAKNLNESLIDLQEL

Sequence Length 36

UniProt ID Q5DIC5

Source Synthetic construct

Activity Information

Target Organism SARS-CoV-2

Assay Cell-cell fusion assay

Activity

[Ref.32047258]SARS-CoV-2:ihibition of cell-cell fusion in Huh-7 cells(IC50=0.18 µM),inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells(IC50=0.98 µM).
[Ref.30989115]SARS-CoV:ihibition of cell-cell fusion in Huh-7 cells(IC50=0.52±0.11 µM),inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells(IC50=2.81 µM);
MERS-CoV:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
HCoV-OC43:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
HCoV-229E:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
HCoV-NL63:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
CoV-WIV1:ihibition of cell-cell fusion in 293T/ACE2 cells(IC50=2.73 µM);
CoV-Rs3367:ihibition of cell-cell fusion in 293T/ACE2 cells(IC50=3.05 µM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity No cytotoxicity information found in the reference(s) presented

Binding Target membrane

Mechanism 2019-nCoV HR1 and HR2 regions are able to interact with each other to form 6-HB and suggest that 2019-nCoV-HR2P may inhibit 2019-nCoV fusion with and entry into the target cell.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C172H292N48O61

Absent amino acids CFHMPTWY

Common amino acids ILN

Mass 4008.5

Pl 4.2

Basic residues 3

Acidic residues 7

Hydrophobic residues 15

Net charge -4

Boman Index -6802

Hydrophobicity -17.78

Aliphatic Index 138.06

Half Life

Mammalian:1.1 hour
Yeast:3 min
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 9

Literature Information

Literature 1

Title A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike.

Pubmed ID 30989115

Reference Sci Adv. 2019 Apr 10;5(4):eaav4580.

Author Xia S, Yan L, Xu W, Agrawal AS, Algaissi A, Tseng CK, Wang Q, Du L, Tan W, Wilson IA, Jiang S, Yang B, Lu L.

DOI 10.1126/sciadv.aav4580

Literature 2

Title Fusion mechanism of 2019-nCoV and fusion inhibitors targeting HR1 domain in spike protein.

Pubmed ID 32047258

Reference Cell Mol Immunol. 2020 Jul;17(7):765-767.

Author Xia S, Zhu Y, Liu M, Lan Q, Xu W, Wu Y, Ying T, Liu S, Shi Z, Jiang S, Lu L.

DOI 10.1038/s41423-020-0374-2