General Information

General Information

DRAVP ID DRAVPe01760

Peptide Name 2019-nCoV-HR2P(SARS-CoV-2-S(1168-1203),SARS-HR2P)

Sequence DISGINASVVNIQKEIDRLNEVAKNLNESLIDLQEL

Sequence Length 36

UniProt ID Q5DIC5

Taxon ID None

Source Synthetic construct

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not available

Activity Information

Target Organism SARS-CoV-2

Assay Cell-cell fusion assay

Activity

[Ref.32047258]SARS-CoV-2:ihibition of cell-cell fusion in Huh-7 cells(IC50=0.18 µM),inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells(IC50=0.98 µM).
[Ref.30989115]SARS-CoV:ihibition of cell-cell fusion in Huh-7 cells(IC50=0.52±0.11 µM),inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells(IC50=2.81 µM);
MERS-CoV:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
HCoV-OC43:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
HCoV-229E:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
HCoV-NL63:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
CoV-WIV1:ihibition of cell-cell fusion in 293T/ACE2 cells(IC50=2.73 µM);
CoV-Rs3367:ihibition of cell-cell fusion in 293T/ACE2 cells(IC50=3.05 µM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity No cytotoxicity information found in the reference(s) presented

Binding Target membrane

Mechanism 2019-nCoV HR1 and HR2 regions are able to interact with each other to form 6-HB and suggest that 2019-nCoV-HR2P may inhibit 2019-nCoV fusion with and entry into the target cell.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C172H292N48O61

Absent amino acids CFHMPTWY

Common amino acids ILN

Mass 4008.5

Pl 4.2

Basic residues 3

Acidic residues 7

Hydrophobic residues 15

Net charge -4

Boman Index -6802

Hydrophobicity -17.78

Aliphatic Index 138.06

Half Life

Mammalian:1.1 hour
Yeast:3 min
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 9

Literature Information

Literature 1

Title A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike.

Pubmed ID 30989115

Reference Sci Adv. 2019 Apr 10;5(4):eaav4580.

Author Xia S, Yan L, Xu W, Agrawal AS, Algaissi A, Tseng CK, Wang Q, Du L, Tan W, Wilson IA, Jiang S, Yang B, Lu L.

DOI 10.1126/sciadv.aav4580

Literature 2

Title Fusion mechanism of 2019-nCoV and fusion inhibitors targeting HR1 domain in spike protein.

Pubmed ID 32047258

Reference Cell Mol Immunol. 2020 Jul;17(7):765-767.

Author Xia S, Zhu Y, Liu M, Lan Q, Xu W, Wu Y, Ying T, Liu S, Shi Z, Jiang S, Lu L.

DOI 10.1038/s41423-020-0374-2