General Information

General Information

DRAVP ID DRAVPe01764

Peptide Name SARS-CoV-2-S(1168–1203)-GSGSGC

Sequence DISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGSGSGC

Sequence Length 42

UniProt ID P0DTC2

Source Synthetic construct

Activity Information

Target Organism SARS-CoV-2

Assay Cell-cell fusion assay,infectivity assay

Activity

[Ref.33082259]SARS-CoV-2:ihibition of cell-cell fusion in 293T cells(IC50=10±8 nM,IC90=98±57 nM),inhibition of infection in Vero E6 cells(IC50~ 6 nM);
SARS-CoV-2_D614G:ihibition of cell-cell fusion in 293T cells(IC50=8±4 nM,IC90=96±50 nM);
SARS-CoV-2_S943P:ihibition of cell-cell fusion in 293T cells(IC50=6±4 nM,IC90=75±42 nM);
SARS-CoV-2_S247R:ihibition of cell-cell fusion in 293T cells(IC50=9±7 nM,IC90=78±59 nM);
MERS-CoV:ihibition of cell-cell fusion in 293T cells(IC50=35±10 nM,IC90>1000 nM),inhibition of infection in Vero E6 cells(IC50~ 3 nM);
SARS-CoV-1:ihibition of cell-cell fusion in 293T cells(IC50=7±5 nM,IC90=43±6 nM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.33082259]Human embryonic kidney HEK293T cells:18% Cytotoxicity at 10 µM;Vero E6 cells:12% Cytotoxicity at 1 µM,30% Cytotoxicity at 10 µM;Human airway epithelial cells:25% Cytotoxicity at 1 µM.

Binding Target membrane

Mechanism The lipopeptide is derived from the C-terminal heptad repeat (HRC) domain of SARS-CoV-2 S that potently inhibits infection by SARS-CoV-2.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C187H316N54O69S

Absent amino acids FHMPTWY

Common amino acids ILNS

Mass 4456.95

Pl 4.2

Basic residues 3

Acidic residues 7

Hydrophobic residues 15

Net charge -4

Boman Index -7072

Hydrophobicity -15.95

Aliphatic Index 118.33

Half Life

Mammalian:1.1 hour
Yeast:3 min
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 15

Literature Information

Literature 1

Title Inhibition of Coronavirus Entry In Vitro and Ex Vivo by a Lipid-Conjugated Peptide Derived from the SARS-CoV-2 Spike Glycoprotein HRC Domain.

Pubmed ID 33082259

Reference mBio. 2020 Oct 20;11(5):e01935-20.

Author Outlaw VK, Bovier FT, Mears MC, Cajimat MN, Zhu Y, Lin MJ, Addetia A, Lieberman NAP, Peddu V, Xie X, Shi PY, Greninger AL, Gellman SH, Bente DA, Moscona A, Porotto M.

DOI 10.1128/mBio.01935-20

Literature 2

Title Intranasal fusion inhibitory lipopeptide prevents direct-contact SARS-CoV-2 transmission in ferrets.

Pubmed ID 33597220

Reference Science. 2021 Mar 26;371(6536):1379-1382.

Author de Vries RD, Schmitz KS, Bovier FT, Predella C, Khao J, Noack D, Haagmans BL, Herfst S, Stearns KN, Drew-Bear J, Biswas S, Rockx B, McGill G, Dorrello NV, Gellman SH, Alabi CA, de Swart RL, Moscona A, Porotto M.

DOI 10.1126/science.abf4896