General Information

General Information

DRAVP ID DRAVPe01773

Peptide Name P8

Sequence SALEEQLKTFLDKFMHELEDLLYQLAL

Sequence Length 27

UniProt ID No entry found

Source Synthetic construct

Activity Information

Target Organism SARS-CoV-2

Assay plaque assay

Activity

[Ref.33580154]SARS-CoV-2:91% inhibition of replication in Vero-E6 cells at 10 μM;inhibition of replication in Calu-3 cells(IC50=46 nM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.33580154]The peptide proved to be devoid of cell toxicity on Vero-E6 and Calu-3 cells.

Binding Target Not found

Mechanism The peptide was designed mimicking the N-terminal helix of hACE2 protein,which could prevent the SARS-CoV-2 from infecting human cells, blocking the interaction between hACE2 and the virus spike protein.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C149H233N33O45S

Absent amino acids CGINPRVW

Common amino acids L

Mass 3238.74

Pl 4.35

Basic residues 3

Acidic residues 6

Hydrophobic residues 12

Net charge -3

Boman Index -2634

Hydrophobicity -1.11

Aliphatic Index 122.96

Half Life

Mammalian:1.9 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 1490

Absorbance 280nm 57.31

Polar residues 3

Literature Information

Literature 1

Title Human ACE2 peptide-mimics block SARS-CoV-2 pulmonary cells infection.

Pubmed ID 33580154

Reference Commun Biol. 2021 Feb 12;4(1):197.

Author Karoyan P, Vieillard V, Gómez-Morales L, Odile E, Guihot A, Luyt CE, Denis A, Grondin P, Lequin O.

DOI 10.1038/s42003-021-01736-8