General Information

General Information

DRAVP ID DRAVPe01778

Peptide Name Alisporivir, Debio-025

Sequence XxXVXAaXXXX

Sequence Length 11

UniProt ID No entry found

Taxon ID None

Source Synthetic construct

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism SARS-CoV-2

Assay plaque assay

Activity

[Ref.32376613]SARS-CoV-2:Inhibition of infection in Vero E6 cells(EC50=0.46±0.04 µM,E90=3.10±1.40 μM).
[Ref.32568027]SARS-CoV-2:Inhibition of infection in Vero E6 cells(EC50=4.9±1.3 µM);
SARS-CoV:Inhibition of infection in Vero E6 cells(EC50=4.3±1.0 µM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.32376613]Vero E6 cells:CC50>20µM.

Binding Target Not found

Mechanism Comment: No comments found on DRAMP database

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Cyclic

N-terminal Modification No specific N-terminal

C-terminal Modification No specific C-terminal

Other Modification The 'X' at position 1 is alpha-aminobutyric acid,position 2 is N-methylalanine,the 'V' at position 3 is N-methylalanine,the 'X' at position 5,8,9 are N-methylleucine,the 'X' at position 10 is N-methylvaline and position 11 is N-methyl-(4R)-4-[(E)-but-2-enyl]-4-methyl-L-threonyl

Stereochemistry Mixed(D-Ala7,D-meth-Ala2)

Physicochemical Information

Formula C8H-2N2O-6

Absent amino acids CDEFGHIKLMNPQRSTWY

Common amino acids X

Mass 0

Pl 0

Basic residues 0

Acidic residues 0

Hydrophobic residues 2

Net charge 0

Boman Index 585

Hydrophobicity 54.55

Aliphatic Index 35.45

Half Life

Mammalian:
Yeast:
E.coli:

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 0

Literature Information

Literature 1

Title Inhibition of SARS-CoV-2 Infection by the Cyclophilin Inhibitor Alisporivir (Debio 025).

Pubmed ID 32376613

Reference Antimicrob Agents Chemother. 2020 Jun 23;64(7):e00876-20.

Author Softic L, Brillet R, Berry F, Ahnou N, Nevers Q, Morin-Dewaele M, Hamadat S, Bruscella P, Fourati S, Pawlotsky JM, Ahmed-Belkacem A.

DOI 10.1128/AAC.00876-20

Literature 2

Title SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology.

Pubmed ID 32568027

Reference J Gen Virol. 2020 Sep;101(9):925-940.

Author Ogando NS, Dalebout TJ, Zevenhoven-Dobbe JC, Limpens RWAL, van der Meer Y, Caly L, Druce J, de Vries JJC, Kikkert M, Bárcena M, Sidorov I, Snijder EJ.

DOI 10.1099/jgv.0.001453