General Information


DRAVP ID  DRAVPe01791

Peptide Name   VG

Sequence  VALDPIDISIVLNKAKSDLEESKEWIRRSNGKLDSIGSGSGC

Sequence Length  42

UniProt ID  No entry found

Source  Synthetic construct



Activity Information


Target Organism  HPIV 3

Assay  Plaque reduction assay

Activity 

  • [Ref.28344321]Human parainfluenza viruse 3(HPIV 3): inhibition of virus infection in Vero cells(IC90=280 ± 247 nM,IC50=1 nM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.28344321]Vero cells: CC50>10000 nM.

Binding Target  membrane

Mechanism  Peptides derived from the HRC of paramyxovirus F proteins interfere with formation of the six-helix bundle in a dominant-negative manner by binding to the transiently exposed HRN coiled coil in the transient fusion intermediate, thereby inhibiting membrane fusion.



Structure Information


PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Acetylation

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C193H323N55O66S

Absent amino acids  FHMQTY

Common amino acids  S

Mass  4502.08

Pl  5.05

Basic residues  6

Acidic residues  7

Hydrophobic residues  14

Net charge  -1

Boman Index  -8108

Hydrophobicity  -32.38

Aliphatic Index  102.14

Half Life 

  •     Mammalian:100 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  5500

Absorbance 280nm  134.15

Polar residues  14



Literature Information


Literature 1

Title   Broad spectrum antiviral activity for paramyxoviruses is modulated by biophysical properties of fusion inhibitory peptides

Pubmed ID   28344321

Reference   Sci Rep. 2017 Mar 8;7:43610.

Author   Mathieu C, Augusto MT, Niewiesk S, Horvat B, Palermo LM, Sanna G, Madeddu S, Huey D, Castanho MA, Porotto M, Santos NC, Moscona A

DOI   10.1038/srep43610