General Information

General Information

DRAVP ID DRAVPe01802

Peptide Name FBP

Sequence RGAHIKGRWKSRCHRF

Sequence Length 16

UniProt ID No entry found

Taxon ID None

Source Synthetic construct

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not available

Activity Information

Target Organism Influenza A virus, Influenza B virus, SARS-CoV-2

Assay Plaque reduction assay

Activity

[Ref.35259078]influenza A virus(H1N1): inhibition of viral multicycle growth in MDCK cells(IC50=3.9 μg/ml);
influenza A virus(H3N2): inhibition of viral replication in MDCK cells(IC50=1.6 μg/ml);
influenza B virus: inhibition of viral replication in MDCK cells(IC50=7.1 μg/ml);
SARS-CoV-2 HKU001a: inhibition of viral replication in Vero E6 cells(IC50=2.9 μg/ml);
SARS-CoV-2 B.1.1.63: inhibition of viral replication in Vero E6 cells(IC50=3.0 μg/ml);
SARS-CoV-2 Delta: inhibition of viral replication in Vero E6 cells(IC50=3.9 μg/ml).

Hemolytic Activity

[Ref.35259078]No significant haemolysis was observed when Turkey red blood cells (RBC) were treated with FBP.

Cytotoxicity

[Ref.35259078]no significant cytotoxicity was detected in MDCK cells treated with 1 mg/ml of FBP.

Binding Target HA

Mechanism FBP could have dual functions: blocked HA-mediated fusion by binding and inhibited endosomal acidification from interfering viral entry by the endocytic pathway.the notable antiviral activity and fusion inhibition activity of FBP on SARS-CoV-2 could be attributed to the inhibition of FBP on endosomal acidification

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C87H139N35O18S

Absent amino acids DELMNPQTVY

Common amino acids R

Mass 1995.34

Pl 12.01

Basic residues 8

Acidic residues 0

Hydrophobic residues 4

Net charge 8

Boman Index -6830

Hydrophobicity -144.38

Aliphatic Index 30.63

Half Life

Mammalian:1 hour
Yeast:2 min
E.coli:2 min

Extinction Coefficient cystines 5500

Absorbance 280nm 366.67

Polar residues 4

Literature Information

Literature 1

Title Fusion-inhibition peptide broadly inhibits influenza virus and SARS-CoV-2, including Delta and Omicron variants.

Pubmed ID 35259078

Reference Emerg Microbes Infect. 2022 Dec;11(1):926-937.

Author Zhao H, Meng X, Peng Z, Lam H, Zhang C, Zhou X, Chan JF, Kao RYT, To KK, Yuen KY.

DOI 10.1080/22221751.2022.2051753