General Information

General Information

DRAVP ID DRAVPe01865

Peptide Name PIH

Sequence CGGGGGSLTEINTELLDLEYEMKKLEEVVKKLEESYIDLKEL

Sequence Length 42

UniProt ID No entry found

Source Synthetic construct

Activity Information

Target Organism MERS-CoV

Assay

Activity

[Ref.31099550]MERS-CoV:inhibition of cell-cell fusion between 293T cells and Huh-7 cells(IC50=1.171 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.31099550]PIH displayed no significant cytotoxicity to 293T, Huh-7, and L02 cells even at a high concentration of 100 μM.

Binding Target membrane

Mechanism Inhibits MERS-Cov S protein-mediated cell fusion, which is the major pathway of MERS-CoV-induced host infections.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C207H340N48O72S2

Absent amino acids AFHPQRW

Common amino acids E

Mass 4717.37

Pl 4.32

Basic residues 5

Acidic residues 11

Hydrophobic residues 12

Net charge -6

Boman Index -5973

Hydrophobicity -40.24

Aliphatic Index 106.67

Half Life

Mammalian:1.2 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 2980

Absorbance 280nm 72.68

Polar residues 13

Literature Information

Literature 1

Title Novel Gold Nanorod-Based HR1 Peptide Inhibitor for Middle East Respiratory Syndrome Coronavirus.

Pubmed ID 31099550

Reference ACS Appl Mater Interfaces. 2019 Jun 5;11(22):19799-19807.

Author Huang X, Li M, Xu Y, Zhang J, Meng X, An X, Sun L, Guo L, Shan X, Ge J, Chen J, Luo Y, Wu H, Zhang Y, Jiang Q, Ning X.

DOI 10.1021/acsami.9b04240