General Information

General Information

DRAVP ID DRAVPe01878

Peptide Name Pep19-2.5

Sequence GCKKYRRFRWKFKGKFWFWG

Sequence Length 20

UniProt ID No entry found

Taxon ID None

Source Synthetic construct

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not available

Activity Information

Target Organism HIV, HSV-1, HCV, HBV

Assay luciferase infection assay

Activity

[Ref.22457281]HIV-1 BaL:inhibition of viral entry in 293T cells(IC50=8 µg/ml);
HIV-1 NL4-3:inhibition of viral entry in 293T cells(IC50=16 µg/ml);
HSV-1:inhibition of viral entry in Vero cells(IC50=0.42 µg/ml);
Hepatitis C virus (HCV):inhibition of viral entry in 293T cells(IC50=40 µg/ml);
Hepatitis B virus (HBV):inhibition of viral entry in 293T cells(IC50=1 µg/ml).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.22457281]No significant cytotoxicity to Vero, TZM-bl and Jurkat cells even at a high concentration of 20 μg/mL.

Binding Target membrane

Mechanism SALPs block entry of a variety of human pathogenic enveloped viruses, such as HIV-1, HBV, HCV, and HSV 1 and 2 by blocking heparan sulfate on the host cell plasma membrane, which serves as a the docking molecule for these pathogens.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C135H187N37O22S

Absent amino acids ADEHILMNPQSTV

Common amino acids K

Mass 2712.26

Pl 11.17

Basic residues 8

Acidic residues 0

Hydrophobic residues 7

Net charge 8

Boman Index -4964

Hydrophobicity -122.5

Aliphatic Index 0

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 17990

Absorbance 280nm 946.84

Polar residues 5

Literature Information

Literature 1

Title A new class of synthetic peptide inhibitors blocks attachment and entry of human pathogenic viruses.

Pubmed ID 22457281

Reference J Infect Dis. 2012 Jun;205(11):1654-64.

Author Krepstakies M, Lucifora J, Nagel CH, Zeisel MB, Holstermann B, Hohenberg H, Kowalski I, Gutsmann T, Baumert TF, Brandenburg K, Hauber J, Protzer U.

DOI 10.1093/infdis/jis273