General Information

General Information

DRAVP ID DRAVPe01896

Peptide Name H2-3

Sequence HGLLYFAIFFVAAWHIRGR

Sequence Length 19

UniProt ID No entry found

Taxon ID None

Source Synthetic construct

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism HCV

Assay

Activity

[Ref.26251517]Hepatitis C Virus(HCV):inhibition of viral infection in Huh7.5.1 cells(EC50=0.54 ± 0.08 μM); inhibition of post-infection in Huh7.5.1 cells(EC50=1.09 ± 0.11 μM);H2-3 peptide inactivated HCV(EC50=82.11 nM).

Hemolytic Activity

[Ref.26251517]<10% hemolysis even at 400 μM against human erythrocytes.

Cytotoxicity

[Ref.26251517]Huh7.5.1 cells: CC50=115.99 ± 16.68 μM;
Bel7402 cells:CC50~200 μM;
HeLa cells:CC50~200 μM.

Binding Target Not found

Mechanism No mechanism information found in the reference(s).

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C113H160N30O21

Absent amino acids CDEKMNPQST

Common amino acids AF

Mass 2274.7

Pl 10.84

Basic residues 4

Acidic residues 0

Hydrophobic residues 12

Net charge 4

Boman Index 300

Hydrophobicity 85.26

Aliphatic Index 113.16

Half Life

Mammalian:3.5 hour
Yeast:10 min
E.coli:>10 hour

Extinction Coefficient cystines 6990

Absorbance 280nm 388.33

Polar residues 3

Literature Information

Literature 1

Title A p7 Ion Channel-derived Peptide Inhibits Hepatitis C Virus Infection in Vitro.

Pubmed ID 26251517

Reference J Biol Chem. 2015 Sep 18;290(38):23254-63.

Author Hong W, Lang Y, Li T, Zeng Z, Song Y, Wu Y, Li W, Cao Z.

DOI 10.1074/jbc.M115.662452