General Information

General Information

DRAVP ID DRAVPe01948

Peptide Name Bomidin

Sequence MGRFKRFRKKFKKLFKKLS

Sequence Length 19

UniProt ID P54228

Source Synthetic construct

Activity Information

Target Organism DENV, HSV,SARS-CoV-2

Assay Plaque-formation assay,fluorescent focus assay, ELISA

Activity

[Ref.35663971]Dengue virus 2(DENV2):inhibition of viral infection in Huh7 cells(50% inhibition at 10 μM);
HSV:inhibition of viral infection in Huh7 cells(50% inhibition at 10 μM);
SARS-CoV-2:inhibition of viral infection in Huh7 cells(80% inhibition at 10 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.35663971]Vero E6 cells:CC50=169.6 ± 1.1 µM.

Binding Target membrane

Mechanism bomidin readily disrupted viral membranes (with components similar to those in the endoplasmic reticulum) and bacterial membranes but did not alter the overall structures of the cell membranes

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C118H197N35O21S

Absent amino acids ACDEHINPQTVWY

Common amino acids K

Mass 2474.14

Pl 12.32

Basic residues 10

Acidic residues 0

Hydrophobic residues 6

Net charge 10

Boman Index -6196

Hydrophobicity -112.11

Aliphatic Index 41.05

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 2

Literature Information

Literature 1

Title Bomidin: An Optimized Antimicrobial Peptide With Broad Antiviral Activity Against Enveloped Viruses.

Pubmed ID 35663971

Reference Front Immunol. 2022 May 19;13:851642.

Author Liu R, Liu Z, Peng H, Lv Y, Feng Y, Kang J, Lu N, Ma R, Hou S, Sun W, Ying Q, Wang F, Gao Q, Zhao P, Zhu C, Wang Y, Wu X.

DOI 10.3389/fimmu.2022.851642