General Information

General Information

DRAVP ID DRAVPe01980

Peptide Name LEDGF/p75 (355-377)

Sequence IHAEIKNSLKIDNLDVNRCIEAL

Sequence Length 23

UniProt ID O75475

Source Synthetic construct

Activity Information

Target Organism HIV

Assay IN enzymatic assay

Activity

[Ref.18331842]Human immunodeficiency virus (HIV): Inhibition of integrase activity (3' end processing)(IC50=165±28 µM); inhibition of integrase activity(strand transfer)(IC50=153±28 µM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target Integrase

Mechanism The LEDGF/p75 peptide modestly inhibited IN catalysis and was dependent on IN–DNA assembly. The peptide was also effective at disrupting LEDGF/p75–IN complex formation.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C113H193N33O36S

Absent amino acids FGMPQTWY

Common amino acids I

Mass 2622.03

Pl 5.48

Basic residues 4

Acidic residues 4

Hydrophobic residues 10

Net charge 0

Boman Index -4168

Hydrophobicity -4.78

Aliphatic Index 140

Half Life

Mammalian:20 hour
Yeast:30 min
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 5

Literature Information

Literature 1

Title Inhibitory profile of a LEDGF/p75 peptide against HIV-1 integrase: insight into integrase-DNA complex formation and catalysis.

Pubmed ID 18331842

Reference FEBS Lett. 2008 Apr 30;582(10):1425-30.

Author Al-Mawsawi LQ, Christ F, Dayam R, Debyser Z, Neamati N.

DOI 10.1016/j.febslet.2008.02.076