DRAVP ID  DRAVPe02001

Peptide Name   peptide 3

Sequence  LKMDKLTLKGMSY

Sequence Length  13

UniProt ID  No entry found

Taxon ID  None

Source  Synthetic construct

Validation   Experimentally Validated

Gene Name/ID  Not Available

GenBank  Not Available

Amino Acid position  Not Available

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not Available

Target Organism  DENV

Assay  Antiviral assays(Cell protection assay, Direct virus inhibition assay, Post-infection assay, Viral attachment assay, Viral entry assay)

Activity  [Ref.36739680]peptide 3 showed the most promising cell protection effects against DENV-1 (60.28%), DENV(36.23%), DENV-3 (73.85%), and DENV-4 (64.84%) at 200 μM. The IC50 of the cell protection effects of peptide 3 against DENV-3 was determined to be 182.27 μM; peptide 3 demonstrated the most promising antiviral activity during post-infection with good inhibitions of DENV-2 (69.05%) and DENV-3 (61.35%) whereas lower inhibitions were observed against DENV-1 (48.47%) and DENV-4 (34.72%) at 200 μM; Peptide 3 showed the highest inhibitions of plaque formations by DENV-2 (69.05%) and DENV-3 (61.35%); Peptide 3 reduced the RNA copy numbers of DENV-2 and DENV-3 by 43.83% and 50.72%, DENV-1 (31.85%); peptide 3 inhibited DENV-4 attachment to Vero cells by 39.58% but almost no inhibition was observed against DENV-1 (7.62%) and DENV-3 (2.56%).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.36739680]did not exhibit any significant cytotoxic effects towards Vero cells up to 200 μM.

Binding Target  Not found

Mechanism  It is possible that peptide 3 inhibited DENV-2 by inhibiting the virus particle itself and this was supported by the observation that peptide 3 showed good direct virus-inactivating effects against DENV-2.

PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Acetylation

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L

Literature 1

Title   Development of novel antiviral peptides against dengue serotypes 1-4. 

Pubmed ID   36739680

Reference   Virology. 2023 Mar;580:10-27.

Author   Lee MF, Anasir MI, Poh CL. 

DOI   10.1016/j.virol.2023.01.016