DRAVP ID  DRAVPe02002

Peptide Name   Peptide 4

Sequence  TGKFKVVKEIAETQHGTIVIRVQYE

Sequence Length  25

UniProt ID  No entry found

Source  Synthetic construct

Target Organism  DENV

Assay  Antiviral assays(Cell protection assay, Direct virus inhibition assay, Post-infection assay, Viral attachment assay, Viral entry assay)

Activity  [Ref.36739680]peptides 4 demonstrated some cell protection effects against DENV-2 and DENV-4 at 47.38%, inhibitions against the other DENV serotypes were very low; Peptide 4 at 200 μM showed the highest inhibitory effects against DENV-2 (74.26%), but it exhibited very low inhibition of DENV-4 and it could not inhibit DENV-1 and DENV-3 plaque formations; peptide 4 demonstrated the highest inhibitory effects against DENV-1 (80.37%),DENV-2 (44.77%) and DENV-4 (72.22%) during post-infection. The IC50 of the inhibitory effects of peptide 4 against DENV-1 during post-infection was determined to be 161.07 μM; Peptide 4 was observed to inhibit DENV-1 and DENV-4 replications significantly with reductions of RNA copy numbers by 63.86% and 70.37%.

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.36739680]did not exhibit any significant cytotoxic effects towards Vero cells up to 200 μM.

Binding Target  Not found

Mechanism  No machanism information found in the reference(s) presented in this entry

PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Acetylation

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L

Literature 1

Title   Development of novel antiviral peptides against dengue serotypes 1-4. 

Pubmed ID   36739680

Reference   Virology. 2023 Mar;580:10-27.

Author   Lee MF, Anasir MI, Poh CL. 

DOI   10.1016/j.virol.2023.01.016