DRAVP ID  DRAVPe02003

Peptide Name   Peptide 5

Sequence  PFGDSYIVIGVGDSALTLHWFRK

Sequence Length  23

UniProt ID  No entry found

Source  Synthetic construct

Target Organism  DENV

Assay  Antiviral assays(Cell protection assay, Direct virus inhibition assay, Post-infection assay, Viral attachment assay, Viral entry assay)

Activity  [Ref.36739680]peptides 5 demonstrated some cell protection effects against DENV-2 and DENV-4 at 59.72%, inhibitions against the other DENV serotypes were very low; exhibited good direct virus-inactivating effects against DENV-2 (64.61%) and DENV-4 (77.08%) whereas lower inhibitions were observed against DENV-1 (16.90%) and DENV-3 (29.80%). The IC50 of the direct virus-inactivating effects of peptide 5 against DENV-4 was determined to be 121.26 μM; peptide 5 demonstrated the highest inhibitory effects against DENV-4 (89.31%) and DENV-2 (19.94%) during post-infection. The IC50 of the inhibition of DENV-4 by peptide 5 during post-infection was determined to be 141.42 μM; Peptide 5 was observed to inhibit DENV-4 replications significantly with reductions of RNA copy numbers by 75.80%.

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.36739680]did not exhibit any significant cytotoxic effects towards Vero cells up to 200 μM.

Binding Target  Not found

Mechanism  peptide 5 could exert virucidal activity by damaging the protein coat of the virus, penetrating the virion, or destroying the viral genome to reduce viral infectivity. 

PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Acetylation

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L

Literature 1

Title   Development of novel antiviral peptides against dengue serotypes 1-4. 

Pubmed ID   36739680

Reference   Virology. 2023 Mar;580:10-27.

Author   Lee MF, Anasir MI, Poh CL. 

DOI   10.1016/j.virol.2023.01.016