General Information


DRAVPR ID  DRAVPR0002

Name   Cholesterol 25-hydroxylase(h25OH)

Sequence 

Sequence Length  272

Source  Homo sapiens (Human)

UniProt ID O95992

Gene CH25H



Activity Information


Target Organism  SARS-CoV-2,VSV

Activity 

  • [Ref.33239446]SARS-CoV-2:The product of Cholesterol 25-hydroxylase, 25-hydroxycholesterol, activates the ER-localized enzyme ACAT to induce internalization of accessible cholesterol on the plasma membrane and restricts SARS-CoV-2 S protein-mediated fusion which inhibits virus replication.(inhibition of VSV-SARS-CoV-2 infection in MA104 cells(EC50=1.49 µM)).



Structure Information


PDB ID  None

Predicted Structure Download  AF-O95992-F1

';

Modification  Glycosylation of Asn at position 5,163,189.



Comment


25HC has been shown to have broad antiviral activity by inhibiting the host cell entry of human immunodeficiency virus (HIV), vesicular stomatitis virus (VSV), Zika virus (ZIKV), Ebola virus (EBOV), Nipah virus (NiV), Russian spring-summer encephalitis virus (RSSEV), porcine viruses (PEDV, PEGV, porcine intestine coronavirus), reovirus, and norovirus. For ZIKV, treatment with 25HC protected mice and rhesus monkeys from ZIKV infection.



Literature Information


Literature 1

Title   Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion. 

Pubmed ID   33239446

Author   Zang R, Case JB, Yutuc E, Ma X, Shen S, Gomez Castro MF, Liu Z, Zeng Q, Zhao H, Son J, Rothlauf PW, Kreutzberger AJB, Hou G, Zhang H, Bose S, Wang X, Vahey MD, Mani K, Griffiths WJ, Kirchhausen T, Fremont DH, Guo H, Diwan A, Wang Y, Diamond MS, Whelan SPJ, Ding S.

Literature 2

Title   Cholesterol 25-Hydroxylase inhibits SARS-CoV-2 and other coronaviruses by depleting membrane cholesterol.

Pubmed ID   32944968

Author   Wang S, Li W, Hui H, Tiwari SK, Zhang Q, Croker BA, Rawlings S, Smith D, Carlin AF, Rana TM.