DRAVPR ID  DRAVPR0054

Name   Shiftless antiviral inhibitor of ribosomal frameshifting protein(RyDEN)

Sequence  MSQEGVELEKSVRRLREKFHGKVSSKKAGALMRKFGSDHTGVGRSIVYGVKQKDGQELSNDLDAQDPPEDMKQDRDIQAVATSLLPLTEANLRMFQRAQDDLIPAVDRQFACSSCDHVWWRRVPQRKEVSRCRKCRKRYEPVPADKMWGLAEFHCPKCRHNFRGWAQMGSPSPCYGCGFPVYPTRILPPRWDRDPDRRSTHTHSCSAADCYNRREPHVPGTSCAHPKSRKQNHLPKVLHPSNPHISSGSTVATCLSQGGLLEDLDNLILEDLKEEEEEEEEVEDEEGGPRE

Sequence Length  291

Source  Homo sapiens (Human)

UniProt ID Q9NUL5

Gene SHFL(C19orf66)

Target Organism  ZIKV,DENV,HCV

Activity 

• [Ref.32150556]Zika virus(ZIKV):C19orf66 interacts and co-localizes with ZIKV nonstructural protein 3 (NS3), thus inducing NS3 degradation via a lysosome-dependent pathway and interrupts the replication of ZIKV.
• [Ref.26735137]Dengue Virus(DENV):RyDEN is likely to interfere with the translation of DENV via interaction with viral RNA and cellular mRNA-binding proteins, resulting in the inhibition of virus replication in infected cells.
• [Ref.32294532]Hepatitis C Virus(HCV):C19orf66 inhibits the replication of HCV by restricting formation of the viral replication organelle.

PDB ID  None

Predicted Structure Download  AF-Q9NUL5-F1

Modification  Acetylation of Ser at position 2(N-acetylserine)

The expression of MARCH2 to be upregulated upon HIV-1 infection. MARCH2 inhibits the production and infection of HIV-1 through ligase activity-dependent envelope protein degradation and/or intracellular retention.

Literature 1

Title   C19orf66 interrupts Zika virus replication by inducing lysosomal degradation of viral NS3.

Pubmed ID   32150556

Author   Wu Y, Yang X, Yao Z, Dong X, Zhang D, Hu Y, Zhang S, Lin J, Chen J, An S, Ye H, Zhang S, Qiu Z, He Z, Huang M, Wei G, Zhu X.

Literature 2

Title   Characterization of RyDEN (C19orf66) as an Interferon-Stimulated Cellular Inhibitor against Dengue Virus Replication.

Pubmed ID   26735137

Author   Suzuki Y, Chin WX, Han Q, Ichiyama K, Lee CH, Eyo ZW, Ebina H, Takahashi H, Takahashi C, Tan BH, Hishiki T, Ohba K, Matsuyama T, Koyanagi Y, Tan YJ, Sawasaki T, Chu JJ, Vasudevan SG, Sano K, Yamamoto N.

Literature 3

Title   C19orf66 is an interferon-induced inhibitor of HCV replication that restricts formation of the viral replication organelle.

Pubmed ID   32294532

Author   Kinast V, Plociennikowska A, Anggakusuma, Bracht T, Todt D, Brown RJP, Boldanova T, Zhang Y, Brüggemann Y, Friesland M, Engelmann M, Vieyres G, Broering R, Vondran FWR, Heim MH, Sitek B, Bartenschlager R, Pietschmann T, Steinmann E.